top of page

Blog

Search

Published: Encapsulation of Bovine Primordial Follicles in Rigid Alginate Does Not Affect Growth Dynamics

  • hannahmcdowell2020
  • Jul 27, 2024
  • 1 min read

Bovine ovaries were used here as a monovulatory model of folliculogenesis to examine the effects of primordial follicle activation and growth under different physical conditions. We found that there were no differences in activation, growth or survival when primordial follicles were cultured in isolation or in situ (remaining in the tissue) under two significantly differently rigid alginate gels. To determine if the extra rigid environment did not affect activation in isolated follicles due to an immediate activation event, we used 5-ethynyl-2′-deoxyuridine (EdU) to track follicle activation during the isolation process. We identified EdU incorporation in granulosa cells after primordial follicles were isolated from the surrounding extracellular matrix (ECM). These findings support that isolation of primordial follicles from the ECM is an activating event and that the differentially rigid environments assessed here had no effect on follicle growth.


 
 
 

Recent Posts

See All
Congratulations, Maya!

During Maya's previous role, she was part of a team that evaluated "changes in self-reported ratings of research abilities among a cohort...

 
 
 

Comments


Featured Posts
Archive
Follow Me
  • Grey Facebook Icon
  • Grey Twitter Icon
  • Grey Instagram Icon
  • Grey Pinterest Icon
bottom of page